Composite

Part:BBa_K3258031:Design

Designed by: Ayush Pandit   Group: iGEM19_Stanford   (2019-10-21)


GFP Flipped


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 209


Design Notes

Designed to create inverted version of the GFP sequence, and result in nonsense RNA.


Source

Synthetic construct.

References